![]() ![]() Flaming is used in wire loops, agar slants, and test tube mouths. Some of the aseptic techniques that are used in laboratories to avoid contamination include flaming and incineration. Additionally, before an inoculum (agar or broth) is picked the inoculating loop should have been totally cooled. To effectively sterilize the inoculating loops, the entire piece of the metallic inoculation wire is heated until it is red hot to ensure complete sterilization has been attained. This requires holding tubes and bottles at a certain slant to reduce the expanse of air-borne microbes that can get into them. Use of the bio-safety cabinet is also essential to prevent aerosols from being released from the laboratory environment. It is also very important to work quickly to avoid exposure to microbes in the atmosphere. Sterilization can be accomplished by cleaning all laboratory equipment, machines, and materials with a 10% solution that has a bleaching effect, washing hands before and after handling laboratory procedures, ensuring that the only materials on the working bench are those that one needs on the desk, keeping test tubes and Petri dishes covered, and using a different sterile wire loop for every different sample. SterilizationĬleaning of surfaces before and after laboratory sessions is termed as sterilization. ![]() ![]() This paper discusses various aseptic techniques used to prevent cross-contamination, the practicality of which is proven by an experiment performed. It is also essential to maintain high standards of sterile environment to ensure that the single species are well isolated from a mix, thus obtaining a pure culture. Additionally, aseptic techniques assist in conserving a pure culture stock while moving the cultures to new media. Some of the benefits of incorporating aseptic techniques in a laboratory include avoiding contamination by foreign bacteria of cultures that are natural in the environment. These include carrying out tests in microbiology, culture transfer, pure culture isolation, and media inoculation. There is a variety of processes that constitute aseptic techniques. ![]()
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